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ARTIGO ORIGINAL

Perfusato autólogo retrógrado no circuito de circulação extracorpórea em pacientes adultos: efeitos sobre a hemodiluição e transfusão de sangue

Ricardo Vieira RegesI; Walter Vilella de Andrade VicenteII; Alfredo José RodriguesIII; Solange BassetoIV; Lafaiete Alves JuniorIV; Adilson Scorzoni FilhoIV; César Augusto FerreiraIV; Paulo Roberto Barbosa ÉvoraV

DOI: 10.5935/1678-9741.20110052

RESUMO

INTRODUÇÃO: Perfusato autólogo retrógrado (PAR) é uma técnica de circulação extracorpórea (CEC) com baixos custos. Estudos anteriores demonstraram que esta técnica reduz a hemodiluição e a necessidade de transfusões de sangue por meio do aumento do hematócrito intraoperatório.
OBJETIVO: Avaliar técnica de PAR em relação à CEC técnica padrão (perfusato cristaloide) em pacientes adultos.
MÉTODOS: Sessenta e dois pacientes foram aleatoriamente alocados em dois grupos: 1) Grupo PAR (n = 27), constituído por pacientes operados utilizando a técnica de PAR e; 2) Grupo Controle, constituído por pacientes operados utilizando técnica padrão de CEC com cristaloides (n = 35). A PAR foi realizada drenando-se o perfusato cristaloide das linhas arterial e venosa, antes da CEC, para uma bolsa coletora de recirculação. Os principais parâmetros analisados foram: 1) parâmetros hemodinâmicos da CEC; 2) valores de hematócrito e hemoglobina; e; 3) necessidade de transfusões de sangue.
RESULTADOS: Observaram-se diferenças estatisticamente significativas de transfusão no intraoperatório e diminuição da hemodiluição em CEC utilizando PAR. Os valores hemodinâmicos durante a CEC foram semelhantes, observando-se tendência de utilização de fluxos menores na CEC dos pacientes do grupo PAR.
CONCLUSÃO: O presente estudo foi projetado em pequena escala para avaliar os efeitos do PAR, o que foi demonstrado em relação aos já conhecidos efeitos na diminuição da hemodiluição em CEC e transfusão sanguínea, porém não mostrou vantagens hemodinâmicas em relação à técnica padrão com perfusato cristaloide.

ABSTRACT

INTRODUCTION: Retrograde autologous priming (RAP) is a cardiopulmonary bypass (CPB) method, at low cost. Previous studies have shown that this method reduces hemodilution and blood transfusions needs through increased intra-operative hematocrit.
OBJECTIVE: To evaluate RAP method, in relation to standard CPB (crystalloid priming), in adult patients.
METHODS: Sixty-two patients were randomly allocated to two groups: 1) Group RAP (n = 27) of patients operated using the RAP and; 2) Control group of patients operated using CPB standard crystalloid method (n = 35). The RAP was performed by draining crystalloid prime from the arterial and venous lines, before CPB, into a collect recycling bag. The main parameters analyzed were: 1) CPB hemodynamic data; 2) Hematocrit and hemoglobin values; 3) The need for blood transfusions.
RESULTS: It was observed statistically significant fewer transfusions during surgery and reduced CPB hemodilution using RAP. The CPB hemodynamic values were similar, observing a tendency to use lower CPB flows in the RAP group patients.
CONCLUSION: This investigation was designed to be a small-scale pilot study to evaluate the effects of RAP, which were demonstrated concerning the CPB hemodilution and blood transfusions.

INTRODUCTION

To reduce the need for homologous blood, and its known risks, the use of crystalloid priming in CPB has been considered the standard practice for decades. This has advantages for blood flow and organ preservation, but results in a mixture with the patient's blood, causing a hemodilution, and, paradoxically, may lead to the need for blood transfusions during surgery. Although this method is widely used as standard CPB, hemodilution is associated with significant mortality and other adverse conditions, especially when the hematocrit reaches values below 20% [1].

In an effort to reduce the effects caused by hemodilution or transfusion requirements in cardiac surgery, several strategies have been proposed including the donation of autologous blood preoperatively or intraoperatively, the use of cell saver to concentrate red blood cells and increased hematocrit, autotransfusion of blood postoperatively lost, and the use of pharmacologic agents to promote clotting after CPB [2,3]. Among these options, studies have shown that routine use of hemofiltration and/or cell saver improves the quality of surgical care [4-6].

Retrograde autologous priming (RAP) is a method of CPB, at low cost, which has been described [1,7] as modification of the method proposed, in 1959, by Panico and Neptune [8]. Previous studies have shown that this method reduces hemodilution during CPB, the reduction of blood transfusions by increasing the hematocrit intraoperatively, especially in patients who have risk factors as anemia, small body surface area, and refuses to receive blood products [1]. This method uses the patient's own blood during of the pump system setting up. Jansen et al. [9] had already shown that reducing the volume it was possible to attenuate the hyperdynamic response after CPB and reported a significant reduction in the use of the blood bank.

This prospective, randomized study was carried out to evaluate the RAP method in relation to CPB standard method, analyzing the hemodynamic values, lactate, hemoglobin and hematocrit, and the need for blood transfusions in adult patients undergoing CABG with CPB. The study does not pretend to be original, it only intended to draw attention to a CPB method, not always remembered, which positively affects two critical parameters: the hemodilution and the transfusion of blood and blood components. It is an inexpensive and effective practice in relation to other more sophisticated methods such as "mini-circuits" and "cell-savers."

 

METHODS

Composition of the groups

The study was approved by the ethical committee of the Hospital of Clinics from the Ribeirão Preto Faculty of Medicine, University of São Paulo, Brazil. A group (RAP group; n=27) of adult patients was operated using the RAP method and another group was operated, without RAP method, using standard crystalloid prime CPB (group control, n=35). Exclusion criteria were age less than 18 years or greater than 85 years, left ventricular ejection fraction smaller than 30% and emergency surgery. In both groups was used membrane oxygenator. All patients were admitted to the intensive care unit and treated as the same standard clinical practice. The physicians responsible for postoperative care of the patients were blinded with respect to the study group.

Method of the retrograde autologous priming (RAP)

The original RAP method, described by Rosengart et al. [7], modified by Eising et al. [10], as follows, was adopted in this investigation (Figure 1). Before RAP starting, mean arterial pressure (MAP) was elevated to approximately 100 mmHg, using small doses of intravenously administered phenylephrine. A recirculation bag was connected to the venous line. The crystalloid priming fluid of the venous reservoir was drained to a minimal level into the bag. The venous side of the circuit was than drained, slowly replacing the crystalloid priming volume by filling the circuit with patient's blood. The recirculation bag was then disconnected from the venous line and connected with the purge line of the arterial filter. The retrograde priming was then continued until the blood volume in the venous reservoir reached approximately 200 mL. This fluid mixture of the venous reservoir was slowly pumped through the membrane oxygenator and the arterial filter, displacing the priming fluid of the tubing, the oxygenator, and the arterial filter into the recirculation bag. The arterial line connecting the patient with the arterial filter was clamped at that time. Finally, the arterial line was drained into the recirculation bag by replacing the crystalloid fluid with the patient's blood. The procedure was performed with the patient's hemodynamics carefully controlled.

 

 

The recirculation bag was then reconnected with the venous reservoir so that crystalloid fluid replacement could be performed during CPB upon hemodynamic requirements. The retrograde priming procedure requires 4 to 5 minutes before the onset of CPB.

The results analysis included: 1) Characteristics and surgical data of the groups RAP and control; 2) CPB hemodynamic data (mean arterial pressure/MAP, blood flow rate/BF, arterial vascular resistance/AVR); 3 total crystalloid infusion and diuresis); 4) Hemoglobin (Hb), hematocrit (Hct), pH and lactate, and; 5) Bleeding and transfusion data.

Statistical analysis

All the data described in the results were analyzed through the T student test, when inside of the normality, or test of Mann-Whitney, when out of the normality. The qui-square test was adopted to analyze the number of transfused bags of blood and derivates. The adopted level of significance was P<0.05.

 

RESULTS

Characteristics and surgical data of the groups RAP and control

Both, RAP and control groups, presented similar clinical-surgical characteristics, which are presented in Table 1.

Mean arterial pressure (MAP) values during CPB were similar in both RAP and control groups (P>0.05) (Table 2, Figure 2A).

 

 

Blood flow (BF) (ml/s) was statistically smaller in the RAP group than the control group (P<0.05), except at the thirty minute when flows were similar (P>0.05) (Table 3, Figure 2B).

 

 

The CPB systemic arterial resistance (dynes.s.c-5) calculated from the relationship between MAP and BF, and was statistically similar (P>0.05) (Table 4, Figure 2C).

 

 

The values of lactate were significantly smaller during CPB in the RAP group in relation to the control (P<0.05) (3.4 ± 0.2 vs. 4.3 ± 0.2 nmol/l), but were similar to previous CPB (1.8 ± 0.2 vs. 1.8 ± 0.1 nmol/l) (P>0.05) and post-CPB (3.5 ± 0.3 vs. 3.6 ± 0.2 mlmol/l) (P>0.05), respectively (Figure 2).

Total CPB crystalloid solution volume and diuresis

The total CPB crystalloid volumes were smaller in the RAP than control group (1131.1 ± 82.4 vs. 2415.1 ± 77.2 ml) (P<0.05). The intraoperative diuresis (755.9 ± 113.6 vs. 1054.1 ± 93.6 ml) and postoperative 24 hours (2239.3 ± 142.9 vs. 2838.2 ± 142.0 ml), were smaller in the RAP group revealing indirectly that the method supplies an economy of priming (Figure 3).

 

 

Clinical and laboratory data

The results chose to reflect the patients intensive care unit evolution were, respectively, the blood levels of urea in the first postoperative day (46.1 ± 4.4 vs. 43.1 ± 4.2 mg/dl) and the internment times (3.1 ± 0.2 vs. 2.8 ± 0.2 days), which were not statistically different in the RAP and control groups (Table 1).

Blood transfusion and hematimetric data

The results showed that there was a tendency, no statistically significant (P>0.05), to a smaller percentage of RAP patients in receiving less blood transfusions (11.1% vs. 17.1%), as well as of plasma (7.4 vs. 14.3%), in the intra-operative, respectively. Among the patients that received blood products in the intraoperative, it was observed a tendency in the RAP group, in receiving less blood (300 ± 0 vs. 500 ± 104.1 ml), as well as of plasma volume transfusion (175 ± 75.0 vs. 430 ± 88.9 ml), respectively. This tendency was not observed so much in relation to the blood transfusion in the postoperative number of patients (37.0 vs. 34.3%) as in the transfused volume (580 ± 80.0 vs. 500 ± 85.3 ml) in those patients that received blood in the RAP group compared to the control patients, respectively. In spite not observed this tendency related to the percentage o patients that received plasma in the postoperative (40.7 vs. 45.7%), the amount of transfused plasma was smaller tendentious in the RAP group, compared to the group control, (384.6 ± 52.9 vs. 637.5 ± 103.6 ml), respectively. All these results are synthesized in Figure 4.

The hemoglobin values before (13.158 ± 0.353 vs. 12.864 ± 0.384 g/dl) and post-CPB (11.225 ± 0.396 vs. 10.521 ± 0.287 g/dl), and hematocrit before (40.408 ± 1.019 vs. 39.675 ± 1.168%) and post-CPB (34.524 ± 1.140 vs. 32.500 ± 0.862%) were similar in the RAP and control groups, as well as the hematocrit in the first postoperative day (32.271 ± 0.757 vs. 33.932 ± 1.051%), respectively (P>0.05). However, during CPB the hemoglobin values (10.654 ± 0.266 vs. 8.818 ± 0.294 g/dl) and hematocrit (32.748 ± 0.778 vs. 27.382 ± 0.887%) were significantly increased (P<0.05) in the RAP group than in the control group, demonstrating the significant reduction of the CPB hemodilution with the use of RAP.

About platelets transfusion, any patient one was transfused in the intra-operative, in both groups, and only 5.7% of the control patients received platelets transfusions in the postoperative. In the RAP group the patients did not received platelets in the postoperative.

 

DISCUSSION

Autotransfusion under the RAP modality has only gained popularity during the last few years. Low hematocrits during CPB are associated with increased risk of in-hospital mortality, intra or postoperative, use of intra-aortic balloon, and return to the pump after initial weaning. The method described has the potential to allow for the perfusionist to lower the hematocrit to a desired level by adding aliquots of clear prime from the prime bags. Another advantage is that most prime displacement occurs in an antegrade direction from the venous line. This contributes to avoid hemodynamic instability at the start of CPB. These advantages were fully observed on the 27 RAP patients included in the study.

The hemodilution associated with the use of an asanguineous (crystalloid) CPB priming results in decreased intraoperative and postoperative hematocrit values in patients undergoing cardiac operations. The current study demonstrated that RAP can safely be used to decrease the crystalloid pump prime and the associate hemodilution. Therefore, the number of patients requiring red blood cell transfusion could be reduced by avoiding the obligatory transfusions triggered by low hematocrit values below minimum safety limit. In the present series of patients this advantage was observed during CPB, and IT was not the case considering the intra-operative as a whole, and in the early postoperative period. Patients undergoing cardiac operations who will require transfusions can be predicted before the operation by a number of variables, including red blood cell mass [11]. This is consistent with the assumption that patients with a low red cell mass are more likely to have low hematocrit values and therefore require transfusions after CPB as a result of the proportionately greater hemodilution caused by CPB in these patients.

As already described in this article, RAP is safe and extremely well tolerated with none morbidity and mortality, related to the method, among patients enrolled in the study. However, there are potential risks described in the literature, and deserve consideration. One potential risk of RAP, paradoxically, is related to its efficiency in minimizing hemodilution in patients undergoing CPB. Early investigators had demonstrated that an inverse linear relationship exists between temperature and blood viscosity, since blood viscosities can increase 10% to 30% under the hypothermic conditions associated with CPB Therefore, moderate hemodilution during CPB has been considered desirable in avoiding this increased viscosity and potential microcirculation damage, and such a degree of hemodilution has generally been provided by a total crystalloid prime [12]. A second theoretical risk of RAP is related to the potential of hemodynamic instability caused by the large volumes of crystalloid solution withdrawn during the RAP process [10]. It is somewhat unexpected in considering the relative hypovolemia induced by RAP that a significant volume or pressor requirement was not subsequently observed in patients subjected to RAP. In fact, withdrawal of RAP volumes was well tolerated, with hemodynamic parameters and pressor requirements that were equivalent to those in control patients. Although some patients require reinfusion of RAP volumes, clinical experience suggested that correction of preoperative dehydration minimized the incidence of RAP reinfusion.

Despite the advantages attributed to the use of RAP few studies were carried out in the last decade. Although recent isolated studies confirm the benefits of recent RAP [13], at least two studies in meta-analysis are not favorable [14,15]. These results suggest that overall.

A literature review and meta-analysis was carried out to assess the clinical effectiveness of retrograde autologous priming of the CPB circuit to reduce allogeneic packed red blood transfusions in adult cardiac surgery. A total of 21,643 patients were identified and eighteen trials were retrieved for full-text review. Six trials met eligibility criteria. Pooled estimates demonstrated that retrograde autologous priming significantly reduced the number of patients receiving intraoperative packed red cell transfusions, total hospital stay packed red cell transfusions, and the number of units transfused of total hospital stay packed red blood cells. Retrograde autologous priming, however, did not provide a clinical benefit in reducing the number of units transfused of intraoperative packed red blood cells. The combined patient population studied in the six trials was mainly primary isolated coronary artery bypass surgery. Assessing the safety of retrograde autologous priming was not possible due to limited data [15]. These observations are consistent with the results obtained in this service experience.

Over the past 10 years there has been a marked decrease of the publications on PAR. It is curious to observe that over the same past 10 years, the concept of minimized CPB has been devised with the same aim of reducing the side effects and strengthening the advantages of standard CPB [16,17]. Alternatively, CPB method with decreased need for blood transfusion, merits citing the increasing routine use of vacuum assisted venous drainage [18].

Finally, the present study was designed to be a small-scale pilot study to evaluate effects of RAP, which could be demonstrated, without any proved hemodynamic advantage. RAP should be further investigated in a larger cohort, preferably in high-risk patients with congestive heart failure, lung disease, or renal failure. RAP has, nowadays, to be compared in terms of cost-effective, with CPB minicircuits already available for clinical use.

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