INTRODUCTION

The incidence of cardiovascular diseases (CVD), which is one of the most important causes of mortality and morbidity in the world, is rapidly increasing. Despite the ongoing developments in their treatment, these diseases are still in the first place as the cause of death in the People’s Republic of China^[1].

A biomarker expressed in the heart tissue and coronary endothelial cells can be released into the blood and pericardial fluid (PF). Since some of the heart-related biomarkers detected in the circulation are expressed in both the heart muscle and skeletal muscle, analyses in the blood may not indicate heart disease. Therefore, besides blood, PF can be used for diagnosis in CVD. The PF within the pericardial cavity, which is the double-walled pouch enveloping the entire heart, shows dynamic changes. The pericardium has immunological, paracrine, vasomotor, and fibrinolytic activity and it affects the myocyte structure, function, and gene expression by synthesizing biomolecules such as eicosanoids (prostaglandin, thromboxane, and leukotriene)^[2]. Recently, the growth Stimulation expressed gene 2 (ST2) (or ınterleukin 1 receptor-like 1 [also known as IL1RL1], T1, Fit1, IL33R, DER4) has been considered as an important prognostic biomarker because of its involvement in the pathogenesis of CVD, its stability, and easy measurement in clinical samples. ST2, which plays an important role in cellular stress and inflammatory response, is the receptor of ınterleukin 33 (IL33)^[3]. IL33, with its cytokine and transcriptional feature, creates a signal between cells as a nuclear factor and helps to protect the heart^[4,5]. IL33, which creates a paracrine effect between cardiac myocytes and fibroblasts when there is mechanical load in the heart, acts by binding to ST2^[4]. ST2 has two important isoforms, these isoforms are membrane-bound ST2 (ST2L or mST2) and soluble ST2 (sST2). ST2L is not free in peripheral circulation. sST2 without transmembrane and intracellular domain moves freely in the peripheral circulation^[5]. While binding of ST2L to IL33 has a cardioprotective effect (mechanical stimulation, decreased cardiac damage, prevention of apoptosis, lowered inflammatory effect, hypertrophy, and fibrosis), binding of sST2 to IL33 causes these positive effects to disappear (Figure 1). sST2 acts as a trap receptor (decoy receptor), reducing the positive effects of IL33^[4].

Fig. 1 - Cardioprotective relationship of interleukin 33 (IL33) with membrane-bound growth STimulation expressed gene 2 (ST2L) and blocking with soluble growth STimulation expressed gene 2 (sST2). DNA=deoxyribonucleic acid.

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Fig. 1 - Cardioprotective relationship of interleukin 33 (IL33) with membrane-bound growth STimulation expressed gene 2 (ST2L) and blocking with soluble growth STimulation expressed gene 2 (sST2). DNA=deoxyribonucleic acid.

High level of sST2 indicates that the heart is under intense stress. This causes cellular death, tissue fibrosis, decreased heart function, and increased rate of disease progression. Therefore, sST2 is considered to be a biomarker of poor prognosis in CVD^[5]. It was found that sST2 rising above the normal level increased many negative conditions due to CVD by approximately three times^[6]. According to multiple clinical studies, sST2 has emerged as a clinically useful prognostic biomarker in patients with CVD, such as myocardial infarction, acute dyspnea, and heart failure, and in the low-risk population^[7].

The main source of sST2 in individuals with healthy and different diseases is currently not fully determined. In a study of neonatal rat heart myocytes related to heart disease, the sST2 in the bloodstream was found to be of myocardial origin^[8]. However, some studies have shown that the predominant source of sST2 in cardiac patients may be vascular endothelial cells rather than the myocardium^[9]. When all the studies are evaluated together, it is revealed that sST2 is produced by cardiac fibroblast, cardiomyocytes, macrovascular (aorta and coronary arteries), and microvascular endothelial cells in case of damage and stress. In another study, parallel increased release of both ST2L and sST2 was detected in cardiomyocytes and cardiac fibroblasts under biomechanical stress^[10]. However, there are no studies on sST2 release in epicardium and pericardium. We assume that two of the sources of sST2 in humans are probably epicardium and pericardium. In coronary artery disease (CAD) patients, research of sST2 only in the blood is not sufficient. For this reason, it is also important to detect sST2 in the PF, the tissue closest to the heart. The fact is that the increase mechanism of ST2, which has a negative effect on the formation of CAD, is not known; there is no ST2 study in the PF in the literature, and unanswered questions about the IL33-ST2 pathway make this study important. Enzyme-linked ımmunosorbent assay (ELISA) kits are often used to measure human sST2 levels in serum and plasma. In this study, differently, gene expression study was performed for the measurement of sST2.

METHODS

Studying Stages of ST2 Gene Expression in PF and Plasma

1. Total ribonucleic acid (RNA) (messenger RNA [mRNA]) Isolation in PF and Plasma

For isolation of mRNA in PF and plasma, Qiagen (Qiagen GmbH, Hilden, Germany) miRNeasy Serum/Plasma Kit (Cat No./ID: 217184) was used. The working procedure of the manufacturer company was followed and total RNA isolation was performed.

2. Complementary deoxyribonucleic acid (cDNA) Obtainment from mRNA in PF and Plasma

In order to transfer mRNA in obtained total RNA to cDNA, RT2 HT First Strand Kit (Qiagen, GmbH, Hilden, Germany, Cat No./ID: 330411) was used. Each reverse transcribe phase was carried out twice. The contents of reverse transcriptase PCR reaction mixture applied to obtain cDNA from mRNA were prepared as in Table 1. According to the work flow chart of the kit, the relevant procedure was followed.

Table 1 - Reverse transcriptase PCR mixture for obtaining cDNA from mRNA.

Ingredients	Amount
RNA	500 ng/µl
Buffer GE (removal of genomic DNA)	2 µl
Ribonuclease-free water	Adjusted to total 10µl

cDNA=complementary deoxyribonucleic acid;

DNA=deoxyribonucleic acid; mRNA=messenger ribonucleic acid; GE= Genomic elution; PCR=polymerase chain reaction;

RNA=ribonucleic acid

Table 1 - Reverse transcriptase PCR mixture for obtaining cDNA from mRNA.

3. Quality and quantity identification from cDNA obtained from PF and plasma

Quality and quantity identification was done by using Thermo Scientific Multiskan Spectrophotometer System (Waltham, Massachusetts, Unite States of America) equipment. Before expression analysis experiments, cDNA quantities obtained from samples were declined to 50 ng/µl for mRNA cDNA.

4. Real-time PCR (QRT-PCR) Method

Before QRT-PCR, mRNA-cDNA preamplification was performed in PF and plasma, and then routine PCR protocol was applied. Rotor Gene 6000 Real-Time PCR Machine (Qiagen GmbH, Hilden, Germany) device and QuantiTect SYBR Green PCR Kit (Qiagen GmbH, Hilden, Germany, Cat No./ID: 204143) were used for QRT-PCR.

Primer pool: 40 µl (20 µl F [10 pmol/µl] + 20 µl R [10 pmol/µl]) primer was used; 210 µl of water was added per primer and the relevant content is given in Table 2.

Table 2 - Primary pool mix for ST2 gene expression.

For 1 sample
5´ miScript master mix	12.5 µl
Taq polimeraz	0.5 µl (250 unit)
Primer pool	7.5 pmol/µl
Total mix: 20.5µl

ST2=growth Stimulation expressed gene 2

Table 2 - Primary pool mix for ST2 gene expression.

The reaction was carried out at 25.5 µl by adding 5 µl cDNA to the total mix. Routine PCR procedure was applied according to Table 3 for sST2 expression level in PF and Plasma.

Table 3 - Routine PCR protocol for ST2 expression level.

Temperature (oC)	Time
95	10 minutes
94	15 seconds	}12 times
60	2 minutes
4

PCR=polymerase chain reaction; ST2=growth STimulation expressed gene 2

Table 3 - Routine PCR protocol for ST2 expression level.

The product obtained after PCR was diluted with 91 µl of water; 5 µl was used for real time PCR (QRT-PCR). For QRT-PCR, the component and volume were used according to Table 4.

Table 4 - QRT-PCR components for sST2 expression level.

Components	Volume
RT2 SYBR® Green qPCR Mastermix	5 µl
F Primer (10 pmol/µl)	0.3 µl
R Primer (10 pmol/µl)	0.3 µl
Ribonuclease/deoxyribonuclease-free water	3.9 µl
cDNA (50 ng/µl)	0,5 µl
Total	10 µl

cDNA=complementary deoxyribonucleic acid; QRT-PCR=quantitative real-time polymerase chain reaction;

sST2=soluble growth STimulation expressed gene 2

Table 4 - QRT-PCR components for sST2 expression level.

The prepared mixture was distributed to the tubes to be 9.5 µl; 0.5 µl cDNA of each sample was added to each tube containing the QRT-PCR premix and the study was performed under the PCR conditions set forth in Table 5.

Table 5 - QRT-PCR settings for ST2.

	Temperature (ºC)	Time
Enzyme activation	95	10 minutes
Denaturation	95	15 seconds	}40 times
Primer binding/elongation	60	60 seconds

QRT-PCR=quantitative real-time polymerase chain reaction; ST2=growth STimulation expressed gene 2

Table 5 - QRT-PCR settings for ST2.

5. Primer Design

In this study, ST2 primers (Metabion International AG/metabion GmbH, Germany) and beta-actin, a housekeeping gene, were used. ST2 primers were designed using the "Gene" interface (http://www.ncbi.nlm.nih.gov/tools/primerblast/) in the National Center for Biotechnology Information database. The sequences of the ST2 and beta-actin primers are shown in Table 6.

Table 6 - ST2 and beta-actin forward and reverse primers.

	Forward primer	Reverse primer
ST2	5'-TTCCAGTAATCGGAGCCCCT-3'	5'-AACCATGTGCAAGGGGAAGAG-3'
Beta-actin	5'-CGTACCACAGGCATTGTGATG-3'	5'-TTTGATGTCACGCACGATTTC-3'

ST2=growth STimulation expressed gene 2

Table 6 - ST2 and beta-actin forward and reverse primers.

RESULTS

As a result of our ELISA study, the sST2 level was found to be 223,992±19,628 ng/ml in PF, and 44,89±4,226 ng/ml and 390,357±25,245 ng/ml in plasma of the control and patient groups (P<0.001) (Figure 2). In our study, the fold changes of sST2 gene expression levels in PF and plasma were evaluated. The fold changes of sST2 gene expression levels in PF and plasma were evaluated with Student’s t-test. As a result, the average Ct value for sST2 was found as 13.33 in PF, and 19.69 and 2.26 in the plasma of the patient and control groups, respectively. As a result, the average Delta (Ct)* value for sST2 was found as 0,790741 in PF, and 0,39037 and -0,7450 in the plasma of the patient and control groups, respectively. While 22^^{(- Ort.Delta CT)} value of sST2 was found as 0.578047 in PF, it was found as 1.31073 and 0.1506 in the patient and control groups’ plasma, respectively. The sST2 fold change (2^-ΔΔCt) in the plasma of patients was calculated as 2.2675 compared to PF (Figure 3). As a result of statistical analysis, significant changes were found in the replicate 2^^{(- Delta Ct}) values of sST2 gene expression in PF and plasma (P<0.001).

Fig. 2 - Determination of soluble growth STimulation expressed gene 2 (sST2) level of as a biomarker for coronary artery disease (CAD) in pericardial fluid (PF) and plasma.

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Fig. 2 - Determination of soluble growth STimulation expressed gene 2 (sST2) level of as a biomarker for coronary artery disease (CAD) in pericardial fluid (PF) and plasma.

Fig. 3 - Fold change values of soluble growth STimulation expressed gene 2 in pericardial fluid (PF) and plasma of coronary artery disease.

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Fig. 3 - Fold change values of soluble growth STimulation expressed gene 2 in pericardial fluid (PF) and plasma of coronary artery disease.

DISCUSSION

In the American College of Cardiology Foundation/American Heart Association - ACCF/AHA - guide, ST2 was defined as a “novel biomarker” in 2013^[11]. The sST2, a biomarker independent of natriuretic peptides and cardiac troponins, has been accepted as an important biomarker in many diseases such as ST-elevation myocardial ınfarction (STEMI), non-STEMI (or NSTEMI), CAD, heart failure, valvular diseases, cardiomyopathy, coronary bypass, acute cardiac allograft rejection, and acute Kawasaki disease^[12]. According to another study, sST2 shows itself as a strong ischemic marker and is used especially in predicting the etiologies of cardiomyopathy^[11]. Studies have reported that ST2 is not affected by factors such as age, body mass index, and renal dysfunction, and its in vitro stability is high^[13,14].

According to the Framingham Heart Study (FHS) Cohort analysis, the sST2 level gives important information in CAD asymptomatic individuals^[15]. The most important reference values for circulating sST2 were obtained from the FHS. While the reference range of ST2 level was 11-45 ng/mL in men (n=462), sST2 was found to be 9-35 ng/mL in women (n=674)^[15,6].

Dieplinger et al.^[6], while the normal mean value of sST2 was 18 ng/ml, stated that concentrations > 35 ng/ml were at high risk for CVD. In the Penn Heart Failure Study (PHFS), it was stated that high sST2 levels gave a predictive idea about death and heart transplantation in patients with heart failure^[16]. According to the PHFS, patients with sST2 value > 35 ng/ml were found to have 2.8 times more risk of CVD than normal individuals^[17].

Another study reported that heart patients with high sST2 values may have an average of 32% heart failure and 45% death after 11 years^[7]. The increase in the level of sST2 in patients with stable CAD may have been caused by chronic inflammation in atherosclerosis. According to large-scale cohort analysis, sST2 was determined as an independent biomarker for cardiovascular mortality in stable CAD patients. According to a study, long-term mortality has been estimated in stable CAD patients with a sST2 value of 24.6 ng/mL in blood plasma, and it has been reported that the risk of death increases two-fold in people above this value^[18].

According to the researches, only ST2 determined in plasma is insufficient for the correct diagnosis of the disease. The fact that the ST2 increasing mechanism that causes the formation of CAD is unknown and the absence of sST2 and gene expression studies in PF indicate that this study is important. In this study, it was aimed to determine ST2 and its gene expression level in PF of cardiovascular patients. The demographic characteristics of the patients included in the study for this purpose are presented in Table 7.

Table 7 - Demographic characteristics of the control and patient groups included in the study.

As a result of our study, compared to the control group, sST2 level of CAD patients was found to be 8.5 times higher in plasma and 5.5 times in PF.

The sST2 expression results were analyzed statistically both in the PF and plasma of the patient group and in the plasma of the control group. The statistical results of the mean average (AVG) cycle threshold (Ct), AVG Delta Ct, 2^^{(-Avg.(Delta(Ct))}, fold change, and P-values obtained from sST2 are presented in Table 8. As a result of statistical analysis, significant changes were found in the replicate 2^^{(- Delta Ct}) values of sST2 gene expression in PF and plasma (P<0.001).

Table 8 - Statistical results of gene expression of sST2 in PF and plasma.

In our study, the increase in sST2 gene expression and sST2 level in CAD plasma and PF supports the prediction that sST2 value > 35 ng/ml increases the risk of CVD. This is actually an expected situation. In previous studies, it was noted that increased sST2 level, which inhibits the cardioprotective effect of IL33, is a biomarker in the diagnosis of CVD. High level of sST2 in the patient group may play an active role in the progression of the disease by contributing to the pathophysiologic mechanism in patients with CAD. While there are many tissues that increase the circulating level of sST2, the most important factor that increases the level of sST2 in PF is cardiovascular tissues, because sST2 release to PF can only be from the closest heart tissue.

In many previous studies, although sST2 release is known to increase in heart and lung diseases, its release focus and rate are still unknown^[19]. There are studies showing that sST2 protein is secreted after vascular endothelial cells IL-1b, tumor necrosis factor, and phorbol ester stimulation in response to an inflammatory stimulus^[20]. In some studies, it was found that there is an increase in sST2 in plasma of patients with pulmonary artery hypertension^[21]. In another study, ıt was reported that human peripheral blood cells and mast cells derived from mouse bone marrow and stimulated subsequently produce soluble sST2^[22].

CONCLUSION

In conclusion, determining the sST2 level only in plasma may not give very clear results for the reasons mentioned. Therefore, in order to make a real prediction, determining sST2 in both plasma and PF is important for knowing the oscillation focus. The study is consistent with previous studies showing that the main source of increased sST2 is not only the myocardium. Before our study, it was assumed that one of the sST2 sources in humans is probably the pericardium. In our study, the detection of high amounts of sST2 levels and gene expression in the PF of the patient group confirmed this prediction. In addition, the increase in both the level of sST2 gene expression and its amount in PF supports the prediction that it can be used as a prognostic biomarker for heart disease. Knowing the sST2 release foci can contribute to the development of more effective treatment methods, such as suppressing sST2 expression with various treatments or preventing sST2-IL33 binding.